Dataset (5) : bulksoil

Project Overview

Comparative metagenomic, phylogenetic, and physiological analyses of soil microbial communities across nitrogen gradients

   metagenomes   project metadata

Visibility	Public
Static Link	http://metagenomics.anl.gov/linkin.cgi?project=4745

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Description

Terrestrial ecosystems are receiving elevated inputs of nitrogen (N) from anthropogenic sources and understanding how these increases in N availability affect soil microbial communities is critical for predicting the associated effects on belowground ecosystems. We used a suite of approaches to analyze the structure and functional characteristics of soil microbial communities from replicated plots in two long-term N fertilization experiments located in contrasting systems. Pyrosequencing-based analyses of 16S rRNA genes revealed no significant effects of N fertilization on bacterial diversity but significant effects on community composition at both sites; copiotrophic taxa (including members of the Proteobacteria and Bacteroidetes phyla) typically increased in relative abundance in the high N plots with oligotrophic taxa (mainly Acidobacteria) exhibiting the opposite pattern. Consistent with the phylogenetic shifts under N fertilization, shotgun metagenomic sequencing revealed increases in the relative abundances of genes associated with DNA/RNA replication, electron transport, and protein metabolism, increases that could be resolved even with the shallow shotgun metagenomic sequencing conducted here (average of 75,000 reads per sample). We also observed shifts in the catabolic capabilities of the communities across the N gradients that were significantly correlated with the phylogenetic and metagenomic responses, indicating possible linkages between the structure and functioning of soil microbial communities. Overall, our results suggest that N fertilization may, directly or indirectly, induce a shift in the predominant microbial life history strategies, favoring a more active, copiotrophic microbial community, a pattern that parallels the often observed replacement of K-selected with r-selected plant species with elevated N. Study Authors: Noah Fierer, Christian L. Lauber, Kelly S. Ramirez, Jesse Zaneveld, Mark A. Bradford, Rob Knight

Funding Source

-

Contact

Administrative
Noah Fierer (noah.fierer@colorado.edu)
University of Colorado at Boulder
CIRES UCB 216 Boulder CO 80309, USA

Technical

Additional Data

mgrast_id	mgp4745

Metagenomes

There are 16 metagenomes in this project.

MG-RAST ID Metagenome Name  bp Count  Sequence Count  Biome  Feature  Material  Location  Country  Coordinates  Sequence Type  Sequence Method  Download 4449877.3 1_54_RL9 34,126,987 84,858 grassland biome meadow soil bulk soil Cedar Creek Ecosystem Science Reserve, Bethel, MN, USA USA 45.39926, -93.26529 WGS 454 metadata submitted analysis 4449362.3 2_309_RL6 30,469,304 88,074 grassland biome meadow soil bulk soil Kellog Biological Station, Hickory Corners, MI, USA USA 42.44158, -85.3760899999999 WGS 454 metadata submitted analysis 4449363.3 2_401_RL7 23,810,360 69,516 grassland biome meadow soil bulk soil Kellog Biological Station, Hickory Corners, MI, USA USA 42.44158, -85.3760899999999 WGS 454 metadata submitted analysis 4449364.3 2_404_RL8 22,988,021 65,582 grassland biome meadow soil bulk soil Kellog Biological Station, Hickory Corners, MI, USA USA 42.44158, -85.3760899999999 WGS 454 metadata submitted analysis 4449365.3 2_409_RL9 13,736,747 39,365 grassland biome meadow soil bulk soil Kellog Biological Station, Hickory Corners, MI, USA USA 42.44158, -85.3760899999999 WGS 454 metadata submitted analysis 4449357.3 1_45_RL7 25,790,999 64,560 grassland biome meadow soil bulk soil Cedar Creek Ecosystem Science Reserve, Bethel, MN, USA USA 45.39926, -93.26529 WGS 454 metadata submitted analysis 4449356.3 2_201_RL1 16,491,779 49,322 grassland biome meadow soil bulk soil Kellog Biological Station, Hickory Corners, MI, USA USA 42.44158, -85.3760899999999 WGS 454 metadata submitted analysis 4449358.3 2_204_RL2 30,994,869 90,765 grassland biome meadow soil bulk soil Kellog Biological Station, Hickory Corners, MI, USA USA 42.44158, -85.3760899999999 WGS 454 metadata submitted analysis 4449360.3 2_301_RL4 29,380,616 84,770 grassland biome meadow soil bulk soil Kellog Biological Station, Hickory Corners, MI, USA USA 42.44158, -85.3760899999999 WGS 454 metadata submitted analysis 4449359.3 2_209_RL3 21,339,728 62,232 grassland biome meadow soil bulk soil Kellog Biological Station, Hickory Corners, MI, USA USA 42.44158, -85.3760899999999 WGS 454 metadata submitted analysis 4449256.3 1_23_RL4 35,553,938 87,810 grassland biome meadow soil bulk soil Cedar Creek Ecosystem Science Reserve, Bethel, MN, USA USA 45.39926, -93.26529 WGS 454 metadata submitted analysis 4449255.3 1_22_RL3 32,258,894 80,499 grassland biome meadow soil bulk soil Cedar Creek Ecosystem Science Reserve, Bethel, MN, USA USA 45.39926, -93.26529 WGS 454 metadata submitted analysis 4449252.3 1_17_RL2 36,665,161 92,615 grassland biome meadow soil bulk soil Cedar Creek Ecosystem Science Reserve, Bethel, MN, USA USA 45.39926, -93.26529 WGS 454 metadata submitted analysis 4449249.3 1_8_RL1 23,664,198 59,998 grassland biome meadow soil bulk soil Cedar Creek Ecosystem Science Reserve, Bethel, MN, USA USA 45.39926, -93.26529 WGS 454 metadata submitted analysis 4449258.3 1_38_RL5 26,412,606 66,029 grassland biome meadow soil bulk soil Cedar Creek Ecosystem Science Reserve, Bethel, MN, USA USA 45.39926, -93.26529 WGS 454 metadata submitted analysis 4449284.3 1_52_RL8 30,913,425 77,306 grassland biome meadow soil bulk soil Cedar Creek Ecosystem Science Reserve, Bethel, MN, USA USA 45.39926, -93.26529 WGS 454 metadata submitted analysis